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Application of RAPD analysis for identification of Lactococcus lactis subsp. cremoris strains isolated from artisanal cultures.

Authors
  • Samarzija, D
  • Sikora, S
  • Redzepović, S
  • Antunac, N
  • Havranek, J
Type
Published Article
Journal
Microbiological Research
Publisher
Elsevier
Publication Date
Jan 01, 2002
Volume
157
Issue
1
Pages
13–17
Identifiers
PMID: 11911609
Source
Medline
License
Unknown

Abstract

Randomly amplified polymorphic DNA (RAPD) was used for identification of Lactococcus lactis subsp. cremoris strains isolated 40 years ago from various dairy homemade products. Total genomic DNAs from six randomly chosen isolates and the reference strain Lactococcus lactis subsp. cremoris NIZO B64 were amplified using four different 10-mer primers. Although most RAPD fragments were common to all six isolates, a sufficient number of polymorphic fragments were also detected that allowed clear distinction of the isolates and the reference strain. The results indicate that RAPD analysis could be a useful and efficient method to distinguish Lactococcus lactis subsp. cremoris at the strain level and to detect genetic diversity.

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