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[Application of p-aminothiophenol as a SERS reporter in immunoassay].

Authors
  • Jiang, Yun
  • Cui, Yan
  • Gu, Ren-Ao
Type
Published Article
Journal
Guang pu xue yu guang pu fen xi = Guang pu
Publication Date
Jul 01, 2006
Volume
26
Issue
7
Pages
1260–1263
Identifiers
PMID: 17020035
Source
Medline
License
Unknown

Abstract

The adsorption of p-aminothiophenol (PATP) molecule on the gold electrode was investigated. The result suggests that PATP was adsorbed perpendicularly onto the substrate. By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules, the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy (SERS) measurements. The p-aminothiophenol (PATP) was used as SERS-reporter molecule. Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.

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