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Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

Authors
  • Víteček, J.1
  • Petrlová, J.2
  • Petřek, J.1
  • Adam, V.2, 3
  • Havel, L.1
  • Kramer, K. J.4
  • Kizek, R.2
  • 1 Mendel University of Agriculture and Forestry, Department of Plant Biology, Zemědělská 1, Brno, CZ-61300, Czech Republic , Brno (Czechia)
  • 2 Mendel University of Agriculture and Forestry, Department of Chemistry and Biochemistry, Zemědělská 1, Brno, CZ-61300, Czech Republic , Brno (Czechia)
  • 3 Masaryk University, Department of Analytical Chemistry, Faculty of Science, Kotlářská 2, Brno, CZ-61137, Czech Republic , Brno (Czechia)
  • 4 US Department of Agriculture, Grain Marketing and Production Research Center, Agricultural Research Service, Manhattan, KS, 66502, USA , Manhattan (United States)
Type
Published Article
Journal
Biologia Plantarum
Publisher
Kluwer Academic Publishers-Consultants Bureau
Publication Date
Sep 01, 2007
Volume
51
Issue
3
Pages
551–555
Identifiers
DOI: 10.1007/s10535-007-0119-4
Source
Springer Nature
Keywords
License
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Abstract

Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.

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