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Application of fluorescence correlation spectroscopy to study substrate binding in styrene maleic acid lipid copolymer encapsulated ABCG2

Authors
  • Horsey, Aaron J.1
  • Briggs, Deborah A.1
  • Holliday, Nicholas D.1
  • Briddon, Stephen J.1, 2
  • Kerr, Ian D.1
  • 1 School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK
  • 2 Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK
Type
Published Article
Journal
Biochimica et Biophysica Acta. Biomembranes
Publisher
Elsevier
Publication Date
Jun 01, 2020
Volume
1862
Issue
6
Identifiers
DOI: 10.1016/j.bbamem.2020.183218
PMID: 32057756
PMCID: PMC7156912
Source
PubMed Central
Keywords
License
Unknown

Abstract

• ABCG2 is solubilised from HEK293T cells into styrene maleic acid lipid copolymers. • Fluorescence correlation spectroscopy confirms that SMALPs contain an ABCG2 dimer. • Purified SMALP-ABCG2 binds the known ABCG2 substrate BODIPY-prazosin. • SMALP-ABCG2 is amenable to pharmacological measurements of ABCG2 in lipid bilayer patches.

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