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Application of DNA Barcoding in Taxonomy and Phylogeny: An Individual Case of COI Partial Gene Sequencing from Seven Animal Species

Authors
  • Drohvalenko, M.
  • Mykhailenko, A.
  • Rekrotchuk, M.
  • Shpak, L.
  • Shuba, V.
  • Trokhymchuk, R.
  • Utevsky, S.
  • Zinenko, O.
Type
Published Article
Journal
Vestnik Zoologii
Publisher
Sciendo
Publication Date
Oct 01, 2019
Volume
53
Issue
5
Pages
375–384
Identifiers
DOI: 10.2478/vzoo-2019-0034
Source
De Gruyter
Keywords
License
Green

Abstract

A part of the COI mitochondrial barcoding gene was sequenced from seven species of different taxonomical groups: Ambystoma mexicanum (Amphibia, Ambystomatidae), Darevskia lindholmi, Lacerta agilis exigua (Reptilia, Lacertidae), Erinaceus roumanicus (Mammalia, Erinaceidae), Macrobiotus sp. 1 and 2 (Eutardigrada, Macrobiotidae) and Cameraria ohridella (Insecta, Gracillariidae). The sequences were compared with available sequences from databases and positioned on phylogenetic trees when the taxa had not yet been sequenced. The presence of Mexican axolotls in herpetoculture in Ukraine was confirmed. The partial COI genes of the Crimean rock lizard and an eastern sub-species of the sand lizard were sequenced. We demonstrated the presence of two tardigrade mitochondrial lineages of the Macrobiotus hufelandi group in the same sample from the Zeya Natural Reserve in the Far East: one was nearly identical to the Italian M. macrocalix, and the other one is similar to M. persimilis and M. vladimiri. We also confirmed the presence of the invasive haplotype “A” of the horse chestnut leaf miner in Ukraine, in line with the hypothesized route of invasion from Central Europe.

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