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Applicability of different brewhouse technologies and gluten-minimization treatments for the production of gluten-free (barley) malt beers : pilot- to industrial-scale

  • Watson, Hellen
  • Vanderputten, D
  • Van Landschoot, Anita
  • Decloedt, Anneleen
Publication Date
Jan 01, 2019
Ghent University Institutional Archive
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The fate of gluten proteins and (poly)peptides throughout the brewing process of reference and gluten-minimized all-malt beers was monitored on both pilot-scale and industrial-scale. Common process steps such as wort separation, cooking, wort and beer clarification already significantly reduce the mass of gluten proteins (72-99%). Nevertheless, gluten derived (poly)peptides remained present at high concentrations in the final reference beers (58-397 ppm). A lauter tun, with course husk material as filter bed, showed to be more effective in reducing the mass of gluten proteins than a mash filter (33% vs. 18%). The mass of gluten proteins and (poly) peptides could be further significantly reduced (16-89% and 33-81% respectively) depending on the use of tannins, AN-PEP (Prolyl-endopeptidase from Aspergillus niger) and silica gel. To render all-barley malt beers gluten-free (<= 20 ppm) (EC No. 41/2009, 2009) gluten-minimization treatments with AN-PEP and silica gel were combined successfully; these beers contained < 5 ppm gluten proteins and < 10 ppm gluten (poly)peptides.

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