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An apparatus for reproducible large scale preparation of polyacrylamide gradient tube gels.

Authors
  • Backhaus, R
  • Schwippert, W
  • Wolf, G
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Feb 01, 1984
Volume
136
Issue
2
Pages
298–300
Identifiers
PMID: 6721133
Source
Medline
Language
English
License
Unknown

Abstract

A device for the preparation of polyacrylamide tube gels for pore gradient gel electrophoresis is described. The glass tubes are arranged in the shape of a hollow cylinder, of which the outer as well as the inner surface can be cooled. By this arrangement, the maximum distance the heat of reaction has to be conducted is 7.5 mm. According to the outer diameter of the tubes, the number of gels held by one device ranges from 125 (5 mm) to 500 (2.5 mm). The gels are demonstrated to be identical, and allow for highly reproducible separations.

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