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The apoptotic efficacy of succinic acid on renal cancer cell lines

Authors
  • Kasarci, Goksu1
  • Ertugrul, Baris1
  • Iplik, Elif Sinem2
  • Cakmakoglu, Bedia1
  • 1 Aziz Sancar Institute of Experimental Medicine, Istanbul University, Capa, Istanbul, Turkey , Istanbul (Turkey)
  • 2 Istanbul Health and Technology University, Istanbul, Turkey , Istanbul (Turkey)
Type
Published Article
Journal
Medical Oncology
Publisher
Springer-Verlag
Publication Date
Oct 23, 2021
Volume
38
Issue
12
Identifiers
DOI: 10.1007/s12032-021-01577-9
Source
Springer Nature
Keywords
Disciplines
  • Original Paper
License
Yellow

Abstract

Recently, studies on the effects of non-toxic substances on cancer prophylaxis have gained value as an alternative to existing treatment options. Current studies have shown that succinic acid or its derivatives exhibit anticancer activity by inducing apoptosis. We aimed to investigate the anticancer activity of succinic acid on renal cancer for the first time in the literature. The cytotoxic activity of succinic acid on CAKI-2 and ACHN as renal cancer cell lines and MRC-5 as a healthy cell line was determined using the WST-1 cytotoxicity test. Apoptotic activity was measured by Annexin V test and cell death ELISA kit. The results showed that 25 μM and 50 μM doses of succinic acid for 24 h remarkably reduced the cell viability for CAKI-2 cells (89.77% and 90.77%) and ACHN cells (41.57% and 54.54%). Also, no significant effect was observed on the healthy cell line, as we expected. Additionally, administration of succinic acid at same doses resulted in apoptotic activity for ACHN cells (19.1 and 12.7) and CAKI-2 cells (19.85 and 29.55). ELISA results with same doses of succinic acid treatment increased the apoptotic fragment rates by 4.7 and 2.13-fold in CAKI-2 cells, and 32.92, 12.7-fold in ACHN cells. Succinic acid is a focal point for cancer treatments not only for its apoptotic success on cancer cells but also for its capacity to be metabolically active for humans. Our results suggest that succinic acid could be a potential therapeutic agent for individual cancer treatment approaches together with further molecular research.

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