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Antitumour Effect of a Mixture of N-Propyl Polysulfides In Vitro

Authors
  • Djordjevic, Dragana1
  • Milovanovic, Jelena2
  • Jurisevic, Milena1
  • Stojanovic, Bojana3
  • Cvetkovic, Olga4
  • Pergal, Marija4
  • Ristanovic, Elizabeta
  • Vojvodic, Danilo
  • Simic, Milos
  • Manojlovic, Dragan5
  • Milovanovic, Marija6
  • Arsenijevic, Nebojsa6
  • 1 Faculty of Medical Sciences, Department of Pharmacy, Serbia , (Serbia)
  • 2 Faculty of Medical Sciences, Department of Histology, Serbia , (Serbia)
  • 3 Faculty of Medical Sciences, Department of Pathophysiology, Serbia , (Serbia)
  • 4 University of Belgrade, Serbia , (Serbia)
  • 5 Faculty of Chemistry, Serbia , (Serbia)
  • 6 Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Serbia , (Serbia)
Type
Published Article
Journal
Serbian Journal of Experimental and Clinical Research
Publisher
Sciendo
Publication Date
Dec 31, 2019
Volume
20
Issue
4
Pages
295–300
Identifiers
DOI: 10.1515/sjecr-2017-0069
Source
De Gruyter
Keywords
License
Green

Abstract

Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays. The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.

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