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Antiplasmodial activity of tick defensins in a mouse model of malaria.

Authors
  • Couto, Joana1
  • Tonk, Miray2
  • Ferrolho, Joana1
  • Antunes, Sandra1
  • Vilcinskas, Andreas3
  • de la Fuente, José4
  • Domingos, Ana5
  • Cabezas-Cruz, Alejandro6
  • 1 Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal. , (Portugal)
  • 2 Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Strasse 2, D-35394 Giessen, Germany. , (Germany)
  • 3 Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Strasse 2, D-35394 Giessen, Germany; Institute for Insect Biotechnology, Justus Liebig University of Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. , (Germany)
  • 4 SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 USA. , (Spain)
  • 5 Global Health and Tropical Medicine - Instituto de Higiene e Medicina, Universidade Nova de Lisboa (GHMT-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal. Electronic address: [email protected] , (Portugal)
  • 6 UMR BIPAR, INRA, ANSES, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, 94700, France; Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic; Institute of Parasitology, Biology Center, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Ticks and tick-borne diseases
Publication Date
May 01, 2018
Volume
9
Issue
4
Pages
844–849
Identifiers
DOI: 10.1016/j.ttbdis.2018.03.011
PMID: 29567145
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Malaria is a mosquito-borne disease affecting millions of people mainly in Sub-Saharan Africa, Asia and some South American countries. Drug resistance to first-line antimalarial drugs (e.g. chloroquine, sulfadoxine-pyrimethamine and artemisinin) is a major constrain in malaria control. Antimicrobial peptides (AMPs) have shown promising results in controlling Plasmodium spp. parasitemia in in vitro and in vivo models of infection. Defensins are AMPs that act primarily by disrupting the integrity of cell membranes of invasive microbes. We previously showed that defensins from the tick Ixodes ricinus inhibited significantly the growth of P. falciparum in vitro, a property that was conserved during evolution. Here, we tested the activity of three I. ricinus defensins against P. chabaudi in mice. A single dose of defensin (120 μl of 1 mg/ml solution) was administered intravenously to P. chabaudi-infected mice, and the parasitemia was followed for 24 h post-treatment. Defensin treatment inhibited significantly the replication (measured as increases in parasitemia) of P. chabaudi after 1 h and 12 h of treatment. Furthermore, defensin injection was not associated with toxicity. These results agreed with the previous report of antiplasmodial activity of tick defensins against P. falciparum in vitro and justify further studies for the use of tick defensins to control malaria. Copyright © 2018 Elsevier GmbH. All rights reserved.

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