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Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice.

Authors
  • Wang, Yang1
  • Esquivel, Rianne2
  • Flingai, Seleeke2
  • Schiller, Zachary A1
  • Kern, Aurélie3
  • Agarwal, Sangya2
  • Chu, Jacqueline2
  • Patel, Ami2
  • Sullivan, Katherine1
  • Wise, Megan C4
  • Broderick, Kate E4
  • Hu, Linden3
  • Weiner, David B2
  • Klempner, Mark S1
  • 1 MassBiologics of University of Massachusetts Medical School, Boston.
  • 2 Vaccine and Immunotherapy Center, Wistar Institute, Philadelphia, Pennsylvania.
  • 3 Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts.
  • 4 Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania.
Type
Published Article
Journal
The Journal of Infectious Diseases
Publisher
Oxford University Press
Publication Date
Mar 15, 2019
Volume
219
Issue
7
Pages
1146–1150
Identifiers
DOI: 10.1093/infdis/jiy627
PMID: 30476132
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of >6 μg/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models. © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected]

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