Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice.
- Authors
- Type
- Published Article
- Journal
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press
- Publication Date
- Mar 15, 2019
- Volume
- 219
- Issue
- 7
- Pages
- 1146–1150
- Identifiers
- DOI: 10.1093/infdis/jiy627
- PMID: 30476132
- Source
- Medline
- Keywords
- Language
- English
- License
- Unknown
Abstract
We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of >6 μg/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models. © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected]
