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Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice

Authors
  • Wang, Yang
  • Esquivel, Rianne
  • Flingai, Seleeke
  • Schiller, Zachary A.
  • Kern, Aurelie
  • Agarwal, Sangya
  • Chu, Jacqueline
  • Patel, Ami
  • Sullivan, Katherine
  • Wise, Megan C.
  • Broderick, Kate E.
  • Hu, Linden
  • Weiner, David B.
  • Klempner, Mark S.
Publication Date
Mar 15, 2019
Source
[email protected]
Keywords
License
Unknown
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Abstract

We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of > 6 mug/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%-77% were protected against an acute challenge by Borrelia-infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models.

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