Affordable Access

Access to the full text

Antimicrobial peptides are present in immune and host defense cells of the human respiratory and gastroinstestinal tracts

Authors
  • Wah, Jenny1
  • Wellek, Anne2
  • Frankenberger, Marion3
  • Unterberger, Pia1
  • Welsch, Ulrich1
  • Bals, Robert2, 4
  • 1 Ludwig Maximilian University, Department of Anatomy, Chair II, München, 80336, Germany , München (Germany)
  • 2 Philipps University Marburg, Hospital of the University of Marburg, Department of Internal Medicine, Division of Pulmonology, Marburg, 35033, Germany , Marburg (Germany)
  • 3 GSF-Institute for Inhalation Biology and Asklepios Fachklinik Gauting, Clinical Cooperation Group “Inflammatory Lung Diseases”, Gauting, 82131, Germany , Gauting (Germany)
  • 4 Hospital of the University of Marburg, Department of Internal Medicine, Division of Pneumonology, Baldingerstrasse 1, Marburg, 35043, Germany , Marburg (Germany)
Type
Published Article
Journal
Cell and Tissue Research
Publisher
Springer-Verlag
Publication Date
Feb 24, 2006
Volume
324
Issue
3
Pages
449–456
Identifiers
DOI: 10.1007/s00441-005-0127-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

Previous studies have implicated antimicrobial peptides in the host defense of the mammalian intestinal and respiratory tract. The aim of the present study has been to characterize further the expression of these molecules in non-epithelial cells of the human pulmonary and digestive systems by detailed immunohistochemical analysis of the small and large bowel and of the large airways and lung parenchyma. Additionally, cells obtained from bronchoalveolar lavage were analyzed by fluorescent activated cell sorting and immunostaining of cytospin preparations. hBD-1, hBD-2, and LL-37 were detected in lymphocytes and macrophages in the large airways, lung parenchyma, duodenum, and colon. Lymphocytes positive for the peptides revealed a staining pattern and distribution that largely matched that of CD3-positive and CD8-positive T-cells. Macrophages with positive staining for the antimicrobial peptides also stained positively for CD68 and CD74. In view of the morphology of the LL-37-positive and hBD-2-positive mucosal lymphocytes, they are probably also B-cells. Thus, antimicrobial peptides of the defensin and cathelicidin families are present in a variety of non-epithelial cells of mucosal organs. These findings confirm that antimicrobial peptides have multiple functions in the biology of the mucosa of these organs.

Report this publication

Statistics

Seen <100 times