Affordable Access

deepdyve-link
Publisher Website

Antimicrobial peptide induced colloidal transformations in bacteria-mimetic vesicles: Combining in silico tools and experimental methods.

Authors
  • Freire, Rafael V M1
  • Pillco-Valencia, Yeny2
  • da Hora, Gabriel C A2
  • Ramstedt, Madeleine3
  • Sandblad, Linda3
  • Soares, Thereza A2
  • Salentinig, Stefan4
  • 1 Department of Chemistry, University of Fribourg, Chemin Du Musée 9, 1700 Fribourg, Switzerland. , (Switzerland)
  • 2 Department of Fundamental Chemistry, Federal University of Pernambuco, Cidade Universitária, 50740-560 Recife, Brazil. , (Brazil)
  • 3 Department of Chemistry, Umeå Center for Microbial Research, Umeå University, SE-901 87 Umeå, Sweden. , (Sweden)
  • 4 Department of Chemistry, University of Fribourg, Chemin Du Musée 9, 1700 Fribourg, Switzerland. Electronic address: [email protected] , (Switzerland)
Type
Published Article
Journal
Journal of Colloid and Interface Science
Publisher
Elsevier
Publication Date
Aug 15, 2021
Volume
596
Pages
352–363
Identifiers
DOI: 10.1016/j.jcis.2021.03.060
PMID: 33839361
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

With the growing challenges of bacteria becoming resistant to conventional antibiotics, antimicrobial peptides (AMPs) may offer a potential alternative. One of the most studied AMPs, the human cathelicidin derived AMP LL-37 is notable for its antimicrobial activity even though its mechanism of action is not fully understood yet. This work investigates the interaction of LL-37 with 1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG) vesicles, which were employed as a bacterial membrane model given the common presence of this phospholipid in the bacterial membrane. Experimental techniques including small angle X-ray scattering, transmission electron microscopy and dynamic light scattering were used to characterize the interactions among LL-37 and POPG. Molecular dynamics simulations complement the experimental studies with molecular-level insights into the process. LL-37 was discovered to actively and critically interact with the POPG vesicles, modifying the membrane curvature that eventually leads to structural transformations from vesicles to mixed micelles. The results shed light on the mechanisms underlying the interactions among LL-37 and bacteria mimetic vesicles and can guide the further development of AMP based antimicrobial materials and therapies. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times