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Antimicrobial ability and mechanism analysis of Lactobacillus species against carbapenemase-producing Enterobacteriaceae.

Authors
  • Chen, Chi-Chung1
  • Lai, Chih-Cheng2
  • Huang, Hui-Ling3
  • Su, Yu-Ting4
  • Chiu, Yu-Hsin5
  • Toh, Han-Siong6
  • Chiang, Shyh-Ren7
  • Chuang, Yin-Ching8
  • Lu, Ying-Chen9
  • Tang, Hung-Jen10
  • 1 Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan; Department of Food Science, National Chiayi University, Chiayi, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 2 Department of Internal Medicine, Kaohsiung Veterans General Hospital Tainan Branch, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 3 Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 4 Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 5 Department of Internal Medicine, Chi Mei Medical Center, Liouying, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 6 Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 7 Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 8 Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan; Department of Internal Medicine, Chi Mei Medical Center, Liouying, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 9 Department of Food Science, National Chiayi University, Chiayi, Taiwan. Electronic address: [email protected] , (Taiwan)
  • 10 Department of Internal Medicine, Chi Mei Medical Center, Tainan, Taiwan. Electronic address: [email protected] , (Taiwan)
Type
Published Article
Journal
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
Publication Date
Jun 01, 2021
Volume
54
Issue
3
Pages
447–456
Identifiers
DOI: 10.1016/j.jmii.2020.01.005
PMID: 32102749
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study aims to investigate the antimicrobial ability and mechanism analysis of Lactobacillus species against carbapenemase-producing Enterobacteriaceae (CPE). Five Lactobacillus spp. strains and 18 CPE clinical isolates were collected. Their anti-CPE effects were assessed by agar well diffusion and broth microdilution assay, as well as time-kill test. Finally, the specific anti-CPE mechanism, especially for the effect of organic acids was determined using broth microdilution method. All of five Lactobacilli isolates displayed the potent activity against most CPE isolates with mean zones of inhibition ranging 10.2-21.1 mm. The anti-CPE activity was not affected by heating, catalase, and proteinase treatment. Under the concentration of 50% LUC0180 cell-free supernatant (CFS), lactic acid, and mix acid could totally inhibit the growth of carbapenem-resistant Klebsiella pneumoniae (CPE0011), and acetic acid could inhibit 67.8%. In contrast, succinic acid and citric acid could not inhibit the growth of CPE0011. While we decreased the concentration to 25%, only lactic acid and mix acid displayed 100% inhibition. In contrast, succinic acid, citric acid and acetic acid did not show any inhibitory effect. Lactobacillus strains exhibit potent anti-CPE activity, and lactic acid produced by Lactobacillus strains is the major antimicrobial mechanism. Copyright © 2020. Published by Elsevier B.V.

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