Affordable Access

Antigen-specific helper T-cell clone supernatant is sufficient to induce both polyclonal proliferation and differentiation of small resting B lymphocytes.

Authors
  • Leclercq, L
  • Bismuth, G
  • Thèze, J
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Oct 01, 1984
Volume
81
Issue
20
Pages
6491–6495
Identifiers
PMID: 6238333
Source
Medline
License
Unknown

Abstract

Gradient-purified resting B lymphocytes can be polyclonally stimulated by antigen-specific major histocompatibility complex (MHC)-restricted helper T lymphocytes as well as by antigen-activated helper T-cell supernatant. In contrast to what has been described so far, we show that helper T-cell supernatant (in the absence of any other added stimulus, such as that provided by anti-mu antibodies) is sufficient to induce both proliferation of resting B cells and their differentiation into IgM-secreting cells. The stimulation induced by the helper T-cell supernatant takes place in serum-free medium and is not MHC-restricted. Our findings strongly support the existence of a B-cell activating factor acting on the resting B cell and causing it to enter the G1 phase of the cell cycle in a MHC-unrestricted manner.

Report this publication

Statistics

Seen <100 times