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Antigen-specific circulating immune complexes in Coxiella burnetii-infected guinea pigs.

Authors
  • Wen, B H1
  • Yu, S R
  • 1 Department of Microbiology, Third Military Medical College, Chongqing, China. , (China)
Type
Published Article
Journal
Experimental and molecular pathology
Publication Date
Oct 01, 1987
Volume
47
Issue
2
Pages
175–184
Identifiers
PMID: 3653345
Source
Medline
License
Unknown

Abstract

An antigen-specific conglutinin-binding assay was developed with artificial immune complexes of Coxiella burnetii and an enzyme-linked immunosorbent assay for the detection of circulating immune complexes. Sera from guinea pigs infected with C. burnetii were examined by this assay, and the percentages of infected guinea pigs with C. burnetii antigen-specific circulating immune complexes were 71, 93, 74, 65, 49, and 27%, respectively, from the first to sixth week after infection. C. burnetii antigen eluted from circulating immune complexes by acid dissociation was detected by counterimmunoelectrophoresis. The antigen-specific conglutinin binding assay was specific, sensitive, and reproducible for assay of circulating immune complexes.

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