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Antigen-specific augmentation factor involved in murine delayed-type footpad reaction. III. Genetic restriction of delayed hypersensitivity augmentation factor (DAF).

Authors
Type
Published Article
Journal
Immunobiology
0171-2985
Publisher
Elsevier
Publication Date
Volume
176
Issue
1-2
Pages
24–34
Identifiers
PMID: 3129361
Source
Medline
License
Unknown

Abstract

We found an antigen-specific factor capable of augmenting delayed-type hypersensitivity (DTH) in the culture supernatant of the mixture of immune T cells and specific antigen, or in the serum of mice immunized with xenogeneic erythrocytes and elicited for DTH footpad reaction. Previous experiments on the genetic restriction of this factor (DTH-augmentation factor; DAF) indicated that DAF activity was effective across the MHC-barrier in C3H/He (H-2k)--BALB/c (H-2d) system. The genetic restriction between DAF and its acceptor cells was then investigated precisely using Igh (immunoglobulin heavy chain locus)-congeneic mice: 1) Expression of DAF activity was MHC-nonrestricted, 2) but was restricted by the Igh-linked gene on the 12th chromosome, 3) such Igh-linked gene restriction was also demonstrated by an absorption test with normal spleen cells. The acceptor cells for DAF were Thy-1+,L3T4+,Lyt-2- T cells.

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