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Antigen presentation in extracellular matrix: interactions of T cells with dendritic cells are dynamic, short lived, and sequential.

Authors
  • Gunzer, M
  • Schäfer, A
  • Borgmann, S
  • Grabbe, S
  • Zänker, K S
  • Bröcker, E B
  • Kämpgen, E
  • Friedl, P
Type
Published Article
Journal
Immunity
Publisher
Elsevier
Publication Date
Sep 01, 2000
Volume
13
Issue
3
Pages
323–332
Identifiers
PMID: 11021530
Source
Medline
License
Unknown

Abstract

Cognate interactions of naive T cells with antigen-presenting dendritic cells require physical cell-cell contacts leading to signal induction and T cell activation. Using a three-dimensional collagen matrix videomicroscopy model for ovalbumin peptide-specific activation of murine and oxidative mitogenesis of human T cells, we show that T cells maintain vigorous migration upon cognate interactions to DC (dendritic cell), continuously crawl across the DC surface, and rapidly detach (median within 6-12 min). These dynamic and short-lived encounters favor sequential contacts with the same or other DC and trigger calcium influx, upregulation of activation markers, T blast formation, and proliferation. We conclude that a tissue environment supports the accumulation of sequential signals, implicating a numeric or "digital" control mechanism for an ongoing primary immune response.

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