Affordable Access

deepdyve-link
Publisher Website

Antigen identification starting from the genome: a "Reverse Vaccinology" approach applied to MenB.

Authors
  • Palumbo, Emmanuelle
  • Fiaschi, Luigi
  • Brunelli, Brunella
  • Marchi, Sara
  • Savino, Silvana
  • Pizza, Mariagrazia
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2012
Volume
799
Pages
361–403
Identifiers
DOI: 10.1007/978-1-61779-346-2_21
PMID: 21993656
Source
Medline
License
Unknown

Abstract

Most of the vaccines available today, albeit very effective, have been developed using traditional "old-style" methodologies. Technologies developed in recent years have opened up new perspectives in the field of vaccinology and novel strategies are now being used to design improved or new vaccines against infections for which preventive measures do not exist. The Reverse Vaccinology (RV) approach is one of the most powerful examples of biotechnology applied to the field of vaccinology for identifying new protein-based vaccines. RV combines the availability of genomic data, the analyzing capabilities of new bioinformatic tools, and the application of high throughput expression and purification systems combined with serological screening assays for a coordinated screening process of the entire genomic repertoire of bacterial, viral, or parasitic pathogens. The application of RV to Neisseria meningitidis serogroup B represents the first success of this novel approach. In this chapter, we describe how this revolutionary approach can be easily applied to any pathogen.

Report this publication

Statistics

Seen <100 times