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Antigen-antibody interactions in capillary electrophoresis.

Authors
Type
Published Article
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
768
Issue
1
Pages
93–103
Identifiers
PMID: 11939562
Source
Medline
License
Unknown

Abstract

Immunoreactions in combination with separations by capillary electrophoresis (CE) are increasingly being used to quantitate specific analytes in biological fluids. Both competitive and non-competitive approaches have been used for the purpose and, in selected cases, now compare favorably with conventional quantitative immunoassays with respect to concentration limits of detection. CE is also a useful method to evaluate antigen-antibody binding on-line and offers unique possibilities for binding constant estimates, also for weakly binding antibodies and antibody fragments. In this review we cover recent developments in the use of antigen-antibody interactions in conjunction with CE and conclude that continued development of miniaturization, on-line preconcentration and more sensitive detection schemes will contribute to the further dissemination of CE-based immunoassays building on already established affinity CE approaches.

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