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Antigastritis effects of Armillariella tabescens (Scop.) Sing. and the identification of its anti-inflammatory metabolites.

Authors
  • Lee, Seulah1
  • Lee, Dahae1, 2
  • Park, Jun Yeon2
  • Seok, Soonja3
  • Jang, Tae Su4
  • Park, Hyun Bong5
  • Shim, Sang Hee6
  • Kang, Ki Sung2
  • Kim, Ki Hyun1
  • 1 School of Pharmacy, Sungkyunkwan University, Suwon, Korea. , (North Korea)
  • 2 College of Korean Medicine, Gachon University, Seongnam, Korea. , (North Korea)
  • 3 Agricultural Microbiology Division, National Institute of Agricultural Sciences, RDA, Iseo-myeon, , Wanju-gun, , Jeollabuk-do, Korea. , (North Korea)
  • 4 Institute of Green Bio Science & Technology, Seoul National University, Pyeong Chang, Korea. , (North Korea)
  • 5 Department of Chemistry, Yale University, New Haven, CT, USA.
  • 6 College of Pharmacy, Duksung Women's University, Dobong-gu, Seoul, Korea. , (North Korea)
Type
Published Article
Journal
The Journal of pharmacy and pharmacology
Publication Date
Mar 01, 2018
Volume
70
Issue
3
Pages
404–412
Identifiers
DOI: 10.1111/jphp.12871
PMID: 29355943
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study demonstrates the biological and chemical analysis of the mushroom Armillariella tabescens (Scop.) Sing. (Tricholomataceae). Chemical structures of the isolates were determined by 1D and 2D NMR, and ESI-MS, as well as comparison with previously reported data. All isolates were tested for anti-inflammatory effects based on their ability to inhibit LPS-stimulated nitric oxide (NO) production in RAW264.7 cells. We found that the MeOH extract of the fruiting bodies of A. tabescens showed antigastritis activity against ethanol-induced gastric damage in rats and notably reduced the gastric damage index compared to control in a concentration-dependent manner. Chemical investigation of the MeOH extract led to the isolation of four steroids (1-4), three alkaloids (5-7), two nucleic acids (8-9) and four fatty acids (10-13). This is the first study to report the identification of all isolates, except for compound 7, from A. tabescens. Compounds 1, 2, 3, 4 and 10 showed inhibition on LPS-stimulated NO production. Treatment with compound 10 inhibited expression of iNOS, COX-2, phospho-IKKα, IKKα, phospho-IκBα, IκBα and NF-kappa B in LPS-stimulated RAW264.7 cells. Compound 10 likely contributes to the health benefits of A. tabescens as an antigastritis agent through its anti-inflammatory effects. © 2018 Royal Pharmaceutical Society.

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