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Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications in cardiovascular disease.

Authors
  • Okada, Tomoyuki
  • Ayada, Kiyoshi
  • Usui, Shinichi
  • Yokota, Kenji
  • Cui, Jinhua
  • Kawahara, Yoshiro
  • Inaba, Tomoki
  • Hirohata, Satoshi
  • Mizuno, Motowo
  • Yamamoto, Daisuke
  • Kusachi, Shozo
  • Matsuura, Eiji
  • Oguma, Keiji
Type
Published Article
Journal
Journal of autoimmunity
Publication Date
Jan 01, 2007
Volume
29
Issue
2-3
Pages
106–115
Identifiers
PMID: 17606364
Source
Medline
License
Unknown

Abstract

Immune responses against heat shock protein 60 (HSP60) of pathogen-origin are thought to be defensive events which, due to molecular mimicry, misdirect to a human counterpart. Therefore, atherosclerosis may be serologically predicted by anti-HSP60 antibodies (Abs). In the present study, we analyzed the clinical prevalence of the serum IgG Abs against Helicobacter pylori (Hp)-derived HSP60 (Hp-HSP60) or its peptide fragments in patients with cardiovascular disease (CVD; n=250), as compared to those in age- and gender-matched non-CVD patients (n=293). Anti-Hp cell lysate Abs frequently appeared in Hp-infected patients who were not associated with CVD. In contrast, Abs against the particular amino acid sequence Hp-HSP60(II3) (II3 region, Glu(141)-Leu(160), in Hp-HSP60) predominantly appeared in CVD patients, as well as IgG anti-human HSP60 (Hu-HSP60(w)). Furthermore, neither titer of anti-Hp-HSP60(II3) nor anti-Hu-HSP60(w) Abs was correlated with the levels of high sensitivity C-reactive protein (hsCRP). This data strongly suggested that IgG anti-Hp-HSP60(II3) Abs cross-reacted with Hu-HSP60(w) were independent diagnostic markers relevant to CVD. Further, the 20 amino acid residues (Glu(141)-Leu(160)) might be predominant CVD-associated epitopes that induce anti-Hu-HSP60 auto-Abs, whose location was predicted in the tertiary structure of Hu-HSP60.

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