Affordable Access

Access to the full text

Anti-Biofilm Properties of Saccharomyces cerevisiae CNCM I-3856 and Lacticaseibacillus rhamnosus ATCC 53103 Probiotics against G. vaginalis

Authors
  • Sabbatini, Samuele1
  • Monari, Claudia1
  • Ballet, Nathalie
  • Decherf, Amélie Cayzeele
  • Bozza, Silvia1
  • Camilloni, Barbara1
  • Perito, Stefano1
  • Vecchiarelli, Anna1
  • 1 (A.V.)
Type
Published Article
Journal
Microorganisms
Publisher
MDPI AG
Publication Date
Aug 24, 2020
Volume
8
Issue
9
Identifiers
DOI: 10.3390/microorganisms8091294
PMID: 32847138
PMCID: PMC7564297
Source
PubMed Central
Keywords
License
Green

Abstract

Bacterial vaginosis (BV) is characterized by the presence of a polymicrobial biofilm where Gardnerella vaginalis plays a key role. Previously, we demonstrated that Saccharomyces cerevisiae CNCM (French National Collection of Cultures of Microorganisms) I-3856 is helpful in resolving experimental simulated BV in mice. In this study, we analyzed its capacity to affect G. vaginalis biofilms and to potentiate the activity of standard antimicrobial agents. We also investigated the anti-biofilm activity of Lacticaseibacillus rhamnosus GG (ATCC 53103), a well-known strain for its intestinal healthy benefits. Biofilm biomass was assessed by crystal violet staining, and G. vaginalis viability was assessed by a colony forming unit (CFU) assay. Here, for the first time, we demonstrated that S. cerevisiae CNCM I-3856 as well as L. rhamnosus GG were able (i) to significantly inhibit G. vaginalis biofilm formation, (ii) to markedly reduce G. vaginalis viability among the biomass constituting the biofilm, (iii) to induce disaggregation of preformed biofilm, and (iv) to kill a consistent amount of bacterial cells in a G. vaginalis preformed biofilm. Furthermore, S. cerevisiae CNCM I-3856 strongly potentiates the metronidazole effect on G. vaginalis biofilm viability. These results suggest that S. cerevisiae CNCM I-3856 as well as L. rhamnosus GG could be potential novel therapeutic agents against bacterial vaginosis.

Report this publication

Statistics

Seen <100 times