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Antibacterial potential of essential oils of Zataria multiflora and Mentha piperita , micro- and nano-formulated forms

Authors
  • Osanloo, Mahmoud1
  • Abdollahi, Abbas2
  • Valizadeh, Alireza3
  • Abedinpour, Niloufar4
  • 1 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran
  • 2 Department of Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
  • 3 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • 4 Department of Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
Type
Published Article
Journal
Iranian Journal of Microbiology
Publisher
Tehran University of Medical Sciences
Publication Date
Feb 01, 2020
Volume
12
Issue
1
Pages
43–51
Identifiers
PMID: 32322379
PMCID: PMC7163042
Source
PubMed Central
Keywords
License
Green

Abstract

Background and Objectives: Plant-derived essential oils (EOs) shave many usages in health and medicine, such as antibacterial agents. The aim of this study was the improvement of antibacterial activities of two EOs using nanotechnology. Materials and Methods: Antibacterial activity was investigated on four important human pathogenic bacteria using the 96-well plate microdilution method, a quantitative approach. Eleven formulations were prepared using each of the EOs. Eventually, the best nanoformulation with the smallest particle size and polydispersive indices (PDI and SPAN) was selected using each EO for further investigations. Moreover, two microemulsions with similar ingredients and the same portion in comparison with two selected nanoemulsions were also prepared. Antibacterial activity of each EO was compared with its micro- and nano-emulsions. Results: The antibacterial efficacy of Zataria multiflora EO (ZMEO) was significantly better than Mentha piperita EO (MPEO). Besides, the antibacterial activity of nanoemulsion of ZMEO with a particle size of 129 ± 12 nm was significantly better than no- and micro-formulated forms of ZMEO. Interestingly, the efficiency of MPEO nanoemulsion (160 ± 25 nm) was also significantly better than MPEO and its micro-formulated form. Conclusion: Regardless of the intrinsic antibacterial property of two examined EOs, by formulating to nanoemulsion, their efficiencies were improved. Nanoemulsion of ZMEO introduced as an inexpensive, potent and green antibacterial agent.

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