Antibacterial gauze is commonly used to prevent wound inflammation and infection. In this study, a novel antibacterial gauze was prepared using antimicrobial peptides and silver nanoparticles, achieving a wide antimicrobial spectrum and increased antimicrobial properties. The properties of the antibacterial gauze were analyzed by scanning electron microscopy, energy-dispersive spectrometry, weight gain rate test, Fourier transform infrared spectroscopy, antibacterial test, and cell activity test. The results of energy-dispersive spectrometry and Fourier transform infrared spectroscopy suggested that the antimicrobial peptides and silver nanoparticles were loaded on the surface of the gauze. The prepared gauze exhibited excellent antibacterial properties against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Bacillus cereus due to the synergistic antibacterial effect of the antimicrobial peptides and silver nanoparticles. Compared with antibacterial gauze loaded with high concentrations of silver nanoparticles, the gauze prepared here has better antimicrobial properties and no obvious cytotoxicity to cells. These results indicate that this antibacterial gauze, with good compatibility, is an attractive candidate for application in wound care and wound healing.