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Anthrax toxin protective antigen--insights into molecular switching from prepore to pore.

Authors
  • Bann, James G
Type
Published Article
Journal
Protein Science
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2012
Volume
21
Issue
1
Pages
1–12
Identifiers
DOI: 10.1002/pro.752
PMID: 22095644
Source
Medline
License
Unknown

Abstract

The protective antigen is a key component of the anthrax toxin, as it allows entry of the enzymatic components edema factor and lethal factor into the host cell, through the formation of a membrane spanning pore. This event is absolutely critical for the pathogenesis of anthrax, and although we have yet to understand the mechanism of pore formation, recent developments have provided key insights into how this process may occur. Based on the available data, a model is proposed for the kinetic steps for protective antigen conversion from prepore to pore. In this model, the driving force for pore formation is the formation of the phi (ϕ)-clamp, a region that forms a leak-free seal around the translocating polypeptide. Formation of the ϕ-clamp elicits movements within the prepore that provide steric freedom for the subsequent conformational changes required to form the membrane spanning pore.

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