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Antagonistic action of diphenylhydantoin and calcium on the level of phosphorylation of particular rat and human brain proteins.

Authors
  • DeLorenzo, R J
Type
Published Article
Journal
Brain Research
Publisher
Elsevier
Publication Date
Sep 23, 1977
Volume
134
Issue
1
Pages
125–138
Identifiers
PMID: 912410
Source
Medline
License
Unknown

Abstract

The opposing actions of diphenylhydantoin (DPH) and calcium on the level of [32P]phosphate incorporation into particular rat and human brain proteins have been demonstrated in this study by employing the techniques of acrylamide gel electrophoresis and autoradiography. In the presence of calcium several brain proteins showed a marked increase in the incorporation of [32P]phosphate from [gamma-32P]ATP. The calcium-induced increase in phosphorylation of two proteins, designated proteins DPH-L and DPH-M, was significantly inhibited by DPH. DPH inhibited both the calcium-stimulated initial rate and net level of [32P]phosphate incorporated into proteins DPH-L and DPH-M in homogenate and synaptosomal preparations. The data presented in this study are compatible with the hypothesis that the effect of DPH on protein phosphorylation may play an important role in mediating the stabilizing actions of this anticonvulsant on neuronal tissue and seizure discharge.

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