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Anoxic media design, preparation, and considerations.

Authors
Type
Published Article
Journal
G Protein Coupled Receptors - Structure
0076-6879
Publisher
Elsevier BV
Publication Date
Volume
397
Pages
3–16
Identifiers
PMID: 16260282
Source
Medline
License
Unknown

Abstract

Exclusion of oxygen from growth media is essential for the growth of anoxic prokaryotes. In general, anaerobic techniques focus on the use of deaerated boiled growth media. Successful enrichment, isolation, and cultivation of anoxic prokaryotes critically depend on the choice of appropriate growth media and incubation conditions. This chapter discusses the requirements of anoxic prokaryotes for growth in the laboratory and different existing methods for their cultivation.

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