Affordable Access

Annexin II stimulates RANKL expression through MAPK.

Authors
  • Li, Fanghong1
  • Chung, Hoyeon
  • Reddy, Sakamuri V
  • Lu, Ganwei
  • Kurihara, Noriyoshi
  • Zhao, Allan Z
  • Roodman, G David
  • 1 Department of Medicine/Hematology-Oncology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Type
Published Article
Journal
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Publication Date
July 2005
Volume
20
Issue
7
Pages
1161–1167
Identifiers
PMID: 15940368
Source
Medline
License
Unknown

Abstract

In addition to upregulating GM-CSF mRNA, AX-II increased RANKL mRNA expression dose-dependently in unfractionated human bone marrow mononuclear cells and modestly increased soluble RANKL in unfractionated human bone marrow mononuclear cell conditioned medium. However, AX-II markedly increased membrane-bound RANKL on human bone marrow stromal cells. Treatment of marrow stromal cells with AX-II activated MAP-kinase (ERKs) and PD 98059 abolished the effect but did not block the increase in GM-CSF. Interestingly, OPG, a natural decoy receptor for RANKL, or anti-GM-CSF partially inhibited OCL formation by AX-II in human bone marrow cells, and the combination of OPG and anti-GM-CSF completely blocked AX-II-induced OCL formation. These data show that AX-II stimulates both the proliferation and differentiation of OCL precursors through production of GM-CSF and RANKL respectively.

Report this publication

Statistics

Seen <100 times