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Androgen Deficiency During Mid- and Late Pregnancy Alters Progesterone Production and Metabolism in the Porcine Corpus Luteum

Authors
  • Grzesiak, Malgorzata1
  • Knapczyk-Stwora, Katarzyna1
  • Ciereszko, Renata E.2, 3
  • Golas, Aniela4
  • Wieciech, Iwona1
  • Slomczynska, Maria1
  • 1 Jagiellonian University in Krakow, Gronostajowa 9, Krakow, 30-387, Poland , Krakow (Poland)
  • 2 University of Warmia and Mazury, Olsztyn, Poland , Olsztyn (Poland)
  • 3 Laboratory of Molecular Diagnostics, University of Warmia and Mazury, Olsztyn, Poland , Olsztyn (Poland)
  • 4 Jagiellonian University in Krakow, Krakow, Poland , Krakow (Poland)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Jun 01, 2014
Volume
21
Issue
6
Pages
778–790
Identifiers
DOI: 10.1177/1933719113518991
Source
Springer Nature
Keywords
License
Yellow

Abstract

We determined whether androgen deficiency induced by flutamide treatment during mid- and late pregnancy affects the functions of the porcine corpus luteum (CL). Pregnant gilts were injected with flutamide between days 43 and 49 (gestation day [GD] 50F), days 83 and 89 (GD90F), or days 101 and 107 (GD108F) of gestation. Antiandrogen treatment increased the luteal progesterone concentration in the GD50F group and decreased progesterone content in the GD90F and GD108F groups. Luteal levels of side-chain cleavage cytochrome P450 (CYP11A1) mRNA and protein were significantly downregulated in the GD90F and GD108F groups as compared with the respective controls. The 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (HSD3B) mRNA and protein expression were significantly reduced only in the GD108F group as compared with the control. Decreased luteal 20α-hydroxysteroid dehydrogenase (AKR1C1) mRNA and protein levels were observed in the GD50F group. Thus, androgen deficiency during pregnancy in pigs led to CL dysfunction that is marked by decreased progesterone production. Furthermore, exposure to flutamide during late pregnancy downregulated steroidogenic enzymes (CYP11A1 and HSD3B) in pigs. We conclude that androgens are important regulators of CL function during pregnancy.

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