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Anatomy and lignification status of the Lophanthera lactescens Ducke plant grown in vitro.

Authors
  • 1
  • 1 Departamento de Produtos Florestais, Instituto de Florestas, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil. , (Brazil)
Type
Published Article
Journal
Brazilian journal of biology = Revista brasleira de biologia
1678-4375
Publication Date
Volume
72
Issue
2
Pages
371–378
Identifiers
PMID: 22735146
Source
Medline
License
Unknown

Abstract

This aim of this study was to evaluate the lignification in young stems of the Lophanthera lactescens Ducke plant grown in vitro L. lactescens (Malpighiaceae), a species endemic in the Brazilian Amazon that possesses both medicinal properties and could be used in the forest product industry. Plants grown in vitro condition in MS medium were analyzed using Infrared (IR) microspectroscopy in a diffuse reflectance mode, fluorescence microscopy. Moreover, histochemical tests such as the Wiesner and Maüle test were run to monitor the process of lignification in cell walls. The spectra of IR microscopy recorded using cross section tissue are representative of guaiacyl/syringyl lignin, based on the 1336 and 1246 cm-1 signal. Individuals presenting stem sprains, resulting from the marked development, produced gelatinous fibers with a clear cellulose layer. Initially, fluorescence microscopy demonstrated lignin deposition in the cell corner region having progressive deposition in the secondary wall of tracheary elements of the stem.

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