Affordable Access

deepdyve-link
Publisher Website

Analytical interference in the therapeutic drug monitoring of methotrexate.

Authors
  • Oudart, Jean-Baptiste1
  • Marquet, Benjamin2
  • Feliu, Catherine3
  • Gozalo, Claire4
  • Djerada, Zoubir3
  • Millart, Hervé3
  • 1 Laboratoire central de biochimie, Hôpital Robert Debré, CHU de Reims, Reims, France, Laboratoire de biochimie médicale et de biologie moléculaire, UMR CNRS 7369, Faculté de médecine, Reims, France. , (France)
  • 2 Laboratoire central de biochimie, Hôpital Robert Debré, CHU de Reims, Reims, France. , (France)
  • 3 Laboratoire de pharmacologie et toxicologie, Hôpital Maison Blanche, CHU de Reims, Reims, France, SFR Cap-Santé-EA3801-Université de Reims, Laboratoire de pharmacologie médicale, Faculté de médecine, Reims, France. , (France)
  • 4 Laboratoire de pharmacologie et toxicologie, Hôpital Maison Blanche, CHU de Reims, Reims, France. , (France)
Type
Published Article
Journal
Annales de biologie clinique
Publication Date
Jun 01, 2016
Volume
74
Issue
3
Pages
333–337
Identifiers
DOI: 10.1684/abc.2016.1135
PMID: 27101631
Source
Medline
Keywords
License
Unknown

Abstract

High-dose of methotrexate chemotherapy is used in the treatment of some tumors. It presents several side effects that required therapeutic drug monitoring, which is commonly performed on 24, 48 and 72h after the beginning of the methotrexate infusion. Treatment of overexposure to methotrexate is based on injection of carboxypeptidase G2, which specifically degrades methotrexate into inactive metabolite: DAMPA. FPIA immunoassay on TDx automated analyzer (Abbott™) was used for therapeutic drug monitoring of methotrexate. This immunoassay presented a significant cross-reactivity between methotrexate and DAMPA, which widely overestimate the residual concentration compared to the gold standard HPLC/MS. TDx automated analyzer was substituted by a new immunoassay on Architect automated analyzer (Abbott™). However, this immunoassay has the same cross-reactivity, which needs to be careful when monitoring methotrexate after an injection of carboxypeptidase G2. In order to determine the most suitable assay for the therapeutic drug monitoring of methotrexate, the knowledge of injection of carboxypeptidase G2 remains essential.

Report this publication

Statistics

Seen <100 times