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Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions.

Authors
  • Sestric, Ryan1
  • Spicer, Vic2
  • Krokhin, Oleg V2
  • Sparling, Richard3
  • Levin, David B1
  • 1 Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada. , (Canada)
  • 2 Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB R3E 3P4, Canada. , (Canada)
  • 3 Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. , (Canada)
Type
Published Article
Journal
FEMS Yeast Research
Publisher
Oxford University Press
Publication Date
Mar 18, 2021
Volume
21
Issue
2
Identifiers
DOI: 10.1093/femsyr/foab007
PMID: 33571365
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Oleaginous yeasts have the ability to store greater than 20% of their mass as neutral lipids, in the form of triacylglycerides. The ATP citrate lyase is thought to play a key role in triacylglyceride synthesis, but the relationship between expression levels of this and other related enzymes is not well understood in the role of total lipid accumulation conferring the oleaginous phenotype. We conducted comparative proteomic analyses with the oleaginous yeast, Yarrowia lipolytica, grown in either nitrogen-sufficient rich media or nitrogen-limited minimal media. Total proteins extracted from cells collected during logarithmic and late stationary growth phases were analyzed by 1D liquid chromatography, followed by mass spectroscopy. The ATP citrate lyase enzyme was expressed at similar concentrations in both conditions, in both logarithmic and stationary phase, but many upstream and downstream enzymes showed drastically different expression levels. In non-lipogenic conditions, several pyruvate enzymes were expressed at higher concentration. These enzymes, especially the pyruvate decarboxylase and pyruvate dehydrogenase, may be regulating carbon flux away from central metabolism and reducing the amount of citrate being produced in the mitochondria. While crucial for the oleaginous phenotype, the constitutively expressed ATP citrate lyase appears to cleave citrate in response to carbon flux upstream from other enzymes creating the oleaginous phenotype. © The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

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