Road-deposited sediments (RDS) were an important environmental medium which particulates accumulated potentially toxic pollutants, ultimately posing a threat to urban water-bodies, and therefore it was important to recognize sources and concentrations of heavy metal. 62 samples from commercial areas, residential areas, intense traffic areas and riverside park respectively in Zhenjiang were analyzed for Zn, Pb, Cu, Cr and Ni. The results indicated remarkably high levels of five metals in the intense traffic area, whilst Zn, Cu, Cr and Ni did not show any discernible variations in other three areas. Principal component analysis was applied to identify the sources of heavy metal contamination. The first factor (source) spanning the greater amount of variance (70%) should be vehicular source or source of mixed origin including industrial and vehicular, and the second factor should be industrial or life sources. The metals are strongly correlated to the amount of organic matter. Finally, concentration enrichment ratio was used to assess degree of metal contamination affected by anthropogenic. The results showed that organic matter was the carrier of heavy metal in RDS and RDS in Zhenjiang had a moderate anthropogenic signals or excess moderate anthropogenic signals.