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Analysis of small and large subunit rDNA introns from several ectomycorrhizal fungi species.

Authors
  • Chen, Li-Hong1
  • Yan, Wei2
  • Wang, Ting1
  • Wang, Yu1
  • Liu, Jian3
  • Yu, Zhuo4
  • 1 College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China. , (China)
  • 2 College of Forestry, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China. , (China)
  • 3 Ordos Institute of Technology, Ordos, Inner Mongolia, China. , (China)
  • 4 College of Agronomy, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China. , (China)
Type
Published Article
Journal
PLoS ONE
Publisher
Public Library of Science
Publication Date
Jan 01, 2021
Volume
16
Issue
3
Identifiers
DOI: 10.1371/journal.pone.0245714
PMID: 33720962
Source
Medline
Language
English
License
Unknown

Abstract

The small (18S) and large (28S) nuclear ribosomal DNA (rDNA) introns have been researched and sequenced in a variety of ectomycorrhizal fungal taxa in this study, it is found that both 18S and 28S rDNA would contain introns and display some degree variation in size, nucleotide sequences and insertion positions within the same fungi species (Meliniomyces). Under investigations among the tested isolates, 18S rDNA has four sites for intron insertions, 28S rDNA has two sites for intron insertions. Both 18S and 28S rDNA introns among the tested isolates belong to group I introns with a set of secondary structure elements designated P1-P10 helics and loops. We found a 12 nt nucleotide sequences TACCACAGGGAT at site 2 in the 3'-end of 28S rDNA, site 2 introns just insert the upstream or the downstream of the12 nt nucleotide sequences. Afters sequence analysis of all 18S and 28S rDNA introns from tested isolates, three high conserved regions around 30 nt nucleotides (conserved 1, conserved 2, conserved 3) and identical nucleotides can be found. Conserved 1, conserved 2 and conserved 3 regions have high GC content, GC percentage is almost more than 60%. From our results, it seems that the more convenient host sites, intron sequences and secondary structures, or isolates for 18S and 28S rDNA intron insertion and deletion, the more popular they are. No matter 18S rDNA introns or 18S rDNA introns among tested isolates, complementary base pairing at the splicing sites in P1-IGS-P10 tertiary helix around 5'-end introns and exons were weak.

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