Affordable Access

Analysis of PTEN and the 10q23 region in primary prostate carcinomas.

Authors
  • Feilotter, H E
  • Nagai, M A
  • Boag, A H
  • Eng, C
  • Mulligan, L M
Type
Published Article
Journal
Oncogene
Publisher
Springer Nature
Publication Date
Apr 02, 1998
Volume
16
Issue
13
Pages
1743–1748
Identifiers
PMID: 9582022
Source
Medline
License
Unknown

Abstract

Deletions involving chromosome 10q23 occur frequently in prostatic carcinomas. Recently, a novel tumour suppressor gene, PTEN, mapping to this interval, has been identified. Mutation or deletion of PTEN has been observed in a proportion of prostate cancer cell lines; however, primary prostate carcinomas have not been studied. We have investigated the involvement of PTEN in primary prostatic adenocarcinomas using a panel of 51 matched normal and prostate tumour DNAs. We first determined the proportion of tumours with allele loss at loci in 10q23 which span the region containing the PTEN gene. Our results show that LOH involving 10q23 is common in primary prostate carcinomas. Twenty-five of 51 (49%) tumours showed loss of heterozygosity (LOH) over the region spanning the PTEN locus. We next directly analysed the PTEN gene for mutations of the coding region using single strand conformation polymorphism (SSCP) and sequence analyses. Of those tumours with LOH, only a single tumour was found to carry a missense mutation in PTEN. No mutations in PTEN were identified in tumours without LOH. Our results suggest either that mutation of PTEN is a late event in prostate tumorigenesis, or that another tumour suppressor gene important in prostate cancer may lie close to PTEN in 10q23.

Report this publication

Statistics

Seen <100 times