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Analysis of polymorphism of 18S rRNA gene in Wuchereria bancrofti microfilariae.

Authors
Type
Published Article
Journal
Microbiology and immunology
Publication Date
Volume
49
Issue
10
Pages
909–914
Identifiers
PMID: 16237268
Source
Medline

Abstract

The polymorphism of the 18S rRNA gene in Wuchereria bancrofti microfilariae (mf) collected from three different zones in India was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The RFLPs of the amplified products obtained after digestion with restriction enzymes Ssp I, Msp I and Hha I showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi.

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