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[Analysis of plasmid pUDKH by anion-exchange high performance liquid chromatogaphy].

Authors
  • Liu, Jia
  • Zhang, Qinglin
  • Bi, Jianjin
  • Wang, Xiaona
  • Gong, Ping
  • Ha, Xiaoqin
  • Wang, Zhengping
  • Wu, Zuze
Type
Published Article
Journal
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui
Publication Date
Jan 01, 2004
Volume
22
Issue
1
Pages
65–67
Identifiers
PMID: 15712951
Source
Medline
License
Unknown

Abstract

pUDKH, a new plasmid DNA comprising human hepatocyte growth factor (HGF), was developed as a potential genetherapeutic product for peripheral arterial disease. An anion-exchange high performance liquid chromatographic (HPLC) method was developed to determine the proportion of plasmid DNA in both opened and supercoiled forms. A TSK gel DNA-NPR column was equilibrated with 50% buffer A (20 mmol/L Tris-HCl, pH 8.8) and 50% buffer B (buffer A with 1 mol/L NaCl added). pUDKH was analyzed by using a gradient HPLC with a mobile phase from 50% B to 80% B and with a UV detection at 260 nm. The flow rate was 0.5 mL/min. The method was also used to monitor the production procedures.

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