Affordable Access

deepdyve-link
Publisher Website

Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq).

Authors
  • Towler, William Ian
  • Church, Jessica D
  • Eshleman, James R
  • Fowler, Mary Glenn
  • Guay, Laura A
  • Jackson, J Brooks
  • Eshleman, Susan H
Type
Published Article
Journal
AIDS Research and Human Retroviruses
Publisher
Mary Ann Liebert
Publication Date
Sep 01, 2008
Volume
24
Issue
9
Pages
1209–1213
Identifiers
DOI: 10.1089/aid.2008.0109
PMID: 18788912
Source
Medline
License
Unknown

Abstract

We analyzed the genetic linkage of nevirapine (NVP) resistance mutations and the genetic complexity of HIV-1 variants in Ugandan infants who were HIV infected despite single dose (SD) prophylaxis. Plasma samples were obtained from six HIV-infected infants who had two or more NVP resistance mutations detected by population sequencing (ViroSeq). ViroSeq PCR products were cloned and transformed, and a single-step amplification-sequencing reaction (AmpliSeq) was used to analyze NVP resistance mutations in cloned HIV-1 variants directly from bacterial colonies. Fifty clones were analyzed for each infant sample. This analysis revealed numerous NVP resistance mutations not detected by population sequencing, genetically linked NVP resistance mutations, and a high degree of genetic complexity at codons that influence NVP susceptibility.

Report this publication

Statistics

Seen <100 times