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Analysis of microglial production of reactive oxygen and nitrogen species.

Authors
  • Neniskyte, Urte
  • Brown, Guy C
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2013
Volume
1041
Pages
103–111
Identifiers
DOI: 10.1007/978-1-62703-520-0_12
PMID: 23813374
Source
Medline
License
Unknown

Abstract

Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

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