Affordable Access

Analysis method of the angiotensin-I converting enzyme inhibitory activity based on micellar electrokinetic chromatography.

Authors
  • Watanabe, Toshiro
  • Mazumder, Tapan Kumar
  • Nagai, Shiro
  • Tsuji, Keisuke
  • Terabe, Shigeru
Type
Published Article
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Publication Date
Jan 01, 2003
Volume
19
Issue
1
Pages
159–161
Identifiers
PMID: 12558042
Source
Medline
License
Unknown

Abstract

A micellar electrokinetic chromatography (MEKC) method was developed for estimating the angiotensin-I converting enzyme (ACE) inhibitory activity by separating the hippuric acid liberated in the ACE reaction mixture in the presence of an inhibitor, captopril. The hippuric acid was successfully separated and detected by MEKC with a 25 mM sodium dodecyl sulfate solution in a 25 mM phosphate-50 mM borate buffer at pH 7.0; the total analysis took about 5 min. A good linear relationship was observed between the inhibitor and the peak area of hippuric acid release. No significant difference in the ACE inhibitory activity (IC50) of captopril (an antihypertensive medicine) or autolyzed-mushrooms (functional foods) was observed between the conventional method and the MEKC method. The MEKC method was found to be a useful technique for a rapid assay of the ACE inhibitory activity.

Report this publication

Statistics

Seen <100 times