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Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages

Authors
  • Tsuboi, Masafumi1
  • Gotoh, Yukiko2, 3
  • 1 Graduate School of Engineering, The University of Tokyo, Tokyo 113-0033, Japan
  • 2 Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan
  • 3 International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo, Tokyo 113-0033, Japan
Type
Published Article
Journal
STAR Protocols
Publisher
Elsevier
Publication Date
Aug 19, 2021
Volume
2
Issue
3
Identifiers
DOI: 10.1016/j.xpro.2021.100763
PMID: 34467231
PMCID: PMC8385115
Source
PubMed Central
Keywords
Disciplines
  • Protocol
License
Unknown

Abstract

Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin immunoprecipitation-qPCR techniques to examine changes in histone H2A ubiquitination patterns at various developmental stages. This protocol can be applied for both in vitro NPCs and NPCs directly isolated from mouse neocortices.

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