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Analysis and Frequency of Bovine Lymphocyte Antigen (BoLA-DRB3) Alleles in Iranian Holstein Cattle

Authors
  • Nassiry, M. R.1
  • Eftekhar Shahroodi, F.1
  • Mosafer, J.1
  • Mohammadi, A.1
  • Manshad, E.1
  • Ghazanfari, S.1
  • Mohammad Abadi, M. R.2, 3
  • Sulimova, G. E.2
  • 1 Ferdowsi University of Mashhad, Center of Excellence in Animal Science, College of Agriculture, Mashhad, 91775-1163, Iran , Mashhad
  • 2 Russian Academy of Science, Vavilov Institute of General Genetics, Moscow, 119991, Russia , Moscow
  • 3 Tabriz University, Department of Animal Science, College of Agriculture, Tabriz, Iran , Tabriz
Type
Published Article
Journal
Russian Journal of Genetics
Publisher
Nauka/Interperiodica
Publication Date
Jun 01, 2005
Volume
41
Issue
6
Pages
664–668
Identifiers
DOI: 10.1007/s11177-005-0142-5
Source
Springer Nature
Keywords
License
Yellow

Abstract

The bovine lymphocyte antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune response by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. DRB3 gene has been extensively evaluated as a candidate marker for association with various bovine diseases and immunological traits. This study describes genetic variability in the BoLA-DRB3 in Iranian Holstein cattle. This is the first study of the DNA polymorphism of the BoLA-DRB3 gene in Iranian Holstein cattle. Hemi-nested PCR-RFLP method is used for identification the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the studied herd (26 alleles). Almost 67% of the alleles were accounted for four alleles (BoLA-DRB3.2*8, *24, *11, and *16) in Iranian Holstein cattle. The DRB3.2*8 allele frequency (26.6%) was higher than the others. The frequencies of the DRB3.2*54, *37, *36, *28, *25, *14, *13, *10, *1 alleles were lower than 1%. Significant distinctions have been found between Iranian Holstein cattle and other cattle breeds studied. In Iranian Holstein cattle the alleles (BoLA-DRB3.2*22, *2, and *16) associated with a lower risk of cystic ovarian disease in Holstein cattle are found. The alleles associated with the resistance to mastitis and to bovine leukemia virus infection BoLA-DRB3.2*11 and *23 are detected with the frequencies 10.4 and 4.4%, respectively. Thus, in the Iranian Holstein cows studied alleles associated with resistance to various diseases are found. The method of DNA-typing of animals can be used in agricultural practice for BoLA-DRB3 allele genotyping of cattle in order to reduce spreading of alleles providing susceptibility to mastitis or leukemia in cattle herds.

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