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Analysis of diuretic doping agents by HPLC screening and GC-MSD confirmation.

Authors
  • Tsai, F Y
  • Lui, L F
  • Chang, B
Type
Published Article
Journal
Journal of Pharmaceutical and Biomedical Analysis
Publisher
Elsevier
Publication Date
Jan 01, 1991
Volume
9
Issue
10-12
Pages
1069–1076
Identifiers
PMID: 1822175
Source
Medline
License
Unknown

Abstract

The simultaneous analysis of 14 diuretics in human urine (belonging to five different pharmacological groups) by reversed-phase high-performance liquid chromatography (HPLC) with a diode-array detector was developed by using a gradient elution with acetonitrile and phosphate buffer containing propylamine hydrochloride on a Bondclone-ODS (10 microns) column. The method was applied to the screening test of several diuretics abused by athletes. The confirmation analysis was performed by gas chromatography-mass spectrometry with ion-selective detection (GC-MSD). The characteristic mass fragment ions obtained by electron-impact (EI) ionization (70 eV) provided sufficient identification of these diuretic agents. During the 1990 weight-lifting contest of Asia in Kao-Hsiung, Republic of China, one urine sample was found positive for the diuretic hydrochlorothiazide.

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