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Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.

Authors
  • Makrantoni, Vasso1
  • Robertson, Daniel1
  • Marston, Adele L2
  • 1 The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • 2 The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK. [email protected]
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2019
Volume
2004
Pages
119–138
Identifiers
DOI: 10.1007/978-1-4939-9520-2_10
PMID: 31147914
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A plethora of biological processes like gene transcription, DNA replication, DNA recombination, and chromosome segregation are mediated through protein-DNA interactions. A powerful method for investigating proteins within a native chromatin environment in the cell is chromatin immunoprecipitation (ChIP). Combined with the recent technological advancement in next generation sequencing, the ChIP assay can map the exact binding sites of a protein of interest across the entire genome. Here we describe a-step-by step protocol for ChIP followed by library preparation for ChIP-seq from yeast cells.

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