Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.
The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
The Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK. [email protected]
- Published Article
Methods in molecular biology (Clifton, N.J.)
- Publication Date
Jan 01, 2019
A plethora of biological processes like gene transcription, DNA replication, DNA recombination, and chromosome segregation are mediated through protein-DNA interactions. A powerful method for investigating proteins within a native chromatin environment in the cell is chromatin immunoprecipitation (ChIP). Combined with the recent technological advancement in next generation sequencing, the ChIP assay can map the exact binding sites of a protein of interest across the entire genome. Here we describe a-step-by step protocol for ChIP followed by library preparation for ChIP-seq from yeast cells.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/31147914