For non-biodegradable volatile organic compounds (VOCs) with low water solubility, the tradition biological method can not achieve a satisfactory removal efficiency, so development of high efficiency pre-treatment technology is a hot issue of research. In this experiment, using poor biodegradable chlorobenzene as the target pollutant and dielectric barrier discharge (DBD) non-thermal plasma as the pretreatment technology for biotrickling filter (BTF) , the effect of DBD on the degradation of chlorobenzene was studied by adjusting the technical parameters of DBD. The effects of the inlet concentration, residence time, humidity and peak voltage on decomposition efficiency were investigated and the decomposition products of chlorobenzene were analyzed. Experimental results showed that DBD could effectively remove waste gaseous chlorobenzene, the removal rate of chlorobenzene increased with the increasing peak voltage. When the peak voltage was ≥ 12kV, less effect of residence time on the degradation of chlorobenzene was found. The optimal humidity range of degradation chlorobenzene was 65% - 75%. Through the analysis of degradation products, the species and concentrations of degradation products increased with the increase of discharge voltage. The products were mainly consisted of organic acids and chlorinated hydrocarbons. The water solubility of degradation products was preferable. Furthermore, with the increase of discharge voltage, the biodegradability of degradation products became higher and higher and the biological toxicity was reduced. It had a promoting effect on the degradation of chlorobenzene when the voltage reached 20 kV. Meanwhile, the O3 concentration increased with the increasing discharge voltage and also enhanced with the rising humidity under the same voltage.