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Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.

Authors
  • Shahian, Tina
  • Narlikar, Geeta J
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2012
Volume
833
Pages
337–349
Identifiers
DOI: 10.1007/978-1-61779-477-3_20
PMID: 22183603
Source
Medline
License
Unknown

Abstract

ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone-DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.

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