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Analysis of calcium signals in steering neuronal growth cones in vitro.

Authors
  • Akiyama, Hiroki
  • Kamiguchi, Hiroyuki
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2014
Volume
1162
Pages
17–27
Identifiers
DOI: 10.1007/978-1-4939-0777-9_2
PMID: 24838955
Source
Medline
License
Unknown

Abstract

Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

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