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Analysis of arsenical metabolites in biological samples.

Authors
Type
Published Article
Journal
Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
Publication Date
Volume
42
Identifiers
DOI: 10.1002/0471140856.tx0433s42
PMID: 20396652
Source
Medline
Keywords
  • [73As]
  • Arsenic
  • Arsenic Oxidation State
  • Hydride Generation-Cryotrapping-Gas Chromatography-Atomic Absorption Spectrometry
  • Methylated Arsenicals
  • Ph-Selective Hydride Generation
  • Thin Layer Chromatography

Abstract

Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.

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